Question
(a) Vectors are DNA molecules that can carry a foreign DNA segment into the host cell.
i) Write the significance of 'ori' in this vector.
ii) Give one example each of vectors used for cloning genes in plants and animals.
(b) Write how can an alien DNA be introduced into
i) an animal cell
ii) a plant cell.
Answer :
(a)
i) ‘ori’ – a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells, this sequences is also responsible for controlling the copy number of the linked DNA. ii) Plants – Agrobacterium tumefaciens Animals – Retrovirus
or
(b) i) Micro injection, Recombinant DNA is directly injected in the nucleus of an animal cell.
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Retrovirus, animal cells are infected with disarmed retrovirus.
ii) Biolistics or Genegun, Plants cell are bombarded with high velocity micro particles of gold or tungsten coated with DNA (rDNA).
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Agrobacterium tumefaciens, delivering gene by disarmed pathogen.
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