Question
(a)
i) Identify and name the structures 'A' and 'B' marked in the image given below :
ii) State their importance in various biotechnology experiments.
or
(b) Explain the process by which a bacterial cell can be made 'competent' to take up foreign DNA from its surroundings, using divalent cations and temperature treatment.
Answer :
(a)
i) ‘A; Circular DNA/Plasmid ‘B’ Bacteriophage
ii) (Plasmid)-Can carry foreign gene into the host cell/acts as cloning vector/has selectable marker/ independent of the control of chromosomal DNA/ high copy number (Bacteriophage) -Cloning vector have the ability to replicate in bacterial cells / independent of the control of chromosomal DNA / high copy number per cell.
or
(b) Treating bacteria with specific concentration of calcium (ions)which increases the efficiency with which DNA enters the bacteria through pores in its cell wall , recombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42°C (heat shock), then putting them back on ice.
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